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- Title
Development and characterization of rabbit proximal tubular epithelial cell lines.
- Authors
Romero, Michael F.; Douglas, Janice G.; Eckert, Richard L.; Hopfer, Ulrich; Jacobberger, James W.; Romero, M F; Douglas, J G; Eckert, R L; Hopfer, U; Jacobberger, J W
- Abstract
We have isolated rabbit kidney proximal tubular epithelial cell lines. The selection was based on their ability to form confluent monolayers on porous supports and to maintain receptor-mediated signal transduction and ion transport, characteristic of the proximal tubule. The isolation method consisted of several steps: (1) superficial cortical proximal tubule segments were microdissected and cultured on a matrix-coated porous support until cells formed a confluent monolayer; (2) primary cultures showing hormone-regulated ion transport typical for the proximal tubule were selected and co-cultured with irradiated fibroblasts; and (3) the epithelial cells surviving after several passages were expanded and passaged on porous substrates. Most of the cell lines developed in this manner were obtained by co-culture with irradiated fibroblasts producing a recombinant retrovirus encoding SV40 large T antigen and G418 resistance. However, SV40 T antigen expression was not essential for immortalization, since neither T antigen nor G418 resistance was detected in the isolated cell lines and co-culture with non-producing 3T3 cells gave similar results. One cell line (vEPT) has been characterized in some detail with respect to morphological, biochemical, and ion transport properties. This line forms confluent monolayers with apical microvilli, tight junctions, and convolutions of the basolateral plasma membrane. Once confluent, monolayers maintain conductances of 25 to 32 mS/cm² for several weeks in culture and possess phlorizin-sensitive short-circuit current (Isc) in glucose containing media, indicative of apical Na+-glucose co-transport. vEPT cells also retain receptor and signaling mechanisms for angiotensin II (Ang II). Apical and basal Ang II and 5,6-epoxy-eicosatrienoic acid (5,6-EET) modulate the Isc in a manner similar to primary cultures. The cell lines share with primary cultures expression of the cytokeratins K8, K10/K11, and K19 (‘nomenclature’ [21]). They also retain several receptor and signal transduction mechanisms. For example, Ang II, arachidonate, bradykinin, 5,6-EET, parathyroid hormone (residues 1 through 34), and purine nucleotides increase cytosolic Ca2+, PTH elevates cAMP levels, and Ang II enhances proximal tubule-specific arachidonic acid metabolism.
- Subjects
CELL culture; CELL lines; MONOMOLECULAR films; BLOOD plasma; CELL membranes; PARATHYROID hormone; BIOCHEMISTRY; CALCIUM metabolism; PROTEIN metabolism; VIRAL antigens; RESEARCH; CYCLIC adenylic acid; BIOLOGICAL transport; ANIMAL experimentation; RESEARCH methodology; KIDNEY tubules; RABBITS; NEOPLASTIC cell transformation; EVALUATION research; MEDICAL cooperation; EPITHELIUM; ELECTRON microscopy; COMPARATIVE studies; TUMOR antigens; EPITHELIAL cells; ARACHIDONIC acid; CELL separation; CYTOLOGY
- Publication
Kidney International, 1992, Vol 42, Issue 5, p1130
- ISSN
0085-2538
- Publication type
journal article
- DOI
10.1038/ki.1992.397