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- Title
Hypotonic stress-induced release of KHCO[sub3] in fused renal epitheloid (MDCK) cells.
- Authors
Kersting, Ulrich; Wojnowski, Leszek; Steigner, Wieland; Oberleithner, Hans
- Abstract
Mechanisms of cell volume regulation induced by the reduction of the osmolality of the Ringer solution by one third were studied in fused Madin-Darby canine kidney (MDCK) cells. Intracellular HCO3-, K+ and Cl- concentrations [ion]i in parallel with cell membrane potential (PD), cell membrane conductance (Gm) and conductances of individual ions [Gmion) were evaluated with microelectrode techniques. Fused cells regulate their cell volume by about 50%. Gm increased from 0.43 ± 0.03 mS/cm² in isotonic Ringer solution to 4.3 ± 0.3 mS/cm² in the steady state phase of cell swelling. GmCl was 0.31 ± 0.03 mS/cm² in isotonic Ringer solution and thus was the dominant individual ion conductance. In hie initial phase of cell swelling GmK, increased transiently 64-fold to 0.32 ± 0.03 mS/cm', and consequently PD hyperpolarized. At peak hyperpolarization GmCl transiently decreased by 15%. Cell swelling increased GmCl 11-fold and GmHCO3 28-fold to 0.95 ± 0.1 mS/cm² in the steady state phase of cell swelling. In this phase GmCl and GmHCO3 were dominating, whereas GmK was only slightly increased compared to isotonic conditions. The hyperpolarization of PD was paralleled by cytoplasm c acidification. At peak acidification [HCO3-]i decreased by 6.4 mmol/kg H2O. Cl-1 extrusion was not detectable in the initial phase of cell swelling. In isotonic Ringer solution [K+]i was 125 ± 5 mmol/kg H2O. During the initial phase of cell swelling 23 ± 5 mmol/kg H2O K+ was extruded, indicating that yet unknown anions participated in cell volume regulation in this phase of cell swelling. In the steady state phase of cell swelling [pH]i was normalized by replenishing [HCO3-]i, whereas Cl- was extruded. We conclude that fused renal epitheloid cells acutely release KHCO3 in response to hypotonicity. but then regain pH homeostasis in the steady state phase of cell swelling.
- Subjects
KIDNEY diseases; CELLS; CELL membranes; MICROELECTRODES; EDEMA
- Publication
Kidney International, 1991, Vol 39, Issue 5, p891
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1991.112