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- Title
Simultaneous Determination of Hydrochlorothiazide and Irbesartan from Pharmaceutical Dosage Forms with RP-HPLC.
- Authors
KURBANOĞLU, Sevinç; YARMAN, Aysu
- Abstract
Objectives: In this work, a simple and rapid liquid chromatographic method for the simultaneous determination of irbesartan (IRBE) and hydrochlorothiazide (HCT) was developed and validated by reverse phase high performance liquid chromatography (RP-HPLC). Materials and Methods: Experimental conditions such as different buffer solutions, various pH values, temperature, composition of the mobile phase, and the effect of flow rate were optimized. Results: The developed RP-HPLC method for these antihypertensive agents was wholly validated and IRBE was detected in the linear range of 0.1-25 μg mL-1 and HCT was detected in the linear range of 0.25-25 μg mL-1. Moreover, the suggested chromatographic technique was successfully applied for the determination of the drugs in human serum and pharmaceutical dosage forms with limit of detection values of 0.008 µg mL-1 for IRBE and 0.012 µg mL-1 for HCT. Conclusion: The proposed rapid analysis method of these antihypertensive drugs can be easily used and applied by pharmaceutical companies for which the analysis time is important.
- Subjects
DOSAGE forms of drugs; IRBESARTAN; HYDROCHLOROTHIAZIDE; HIGH performance liquid chromatography; BUFFER solutions; ANTIHYPERTENSIVE agents
- Publication
Turkish Journal of Pharmaceutical Sciences, 2020, Vol 17, Issue 5, p523
- ISSN
1304-530X
- Publication type
Article
- DOI
10.4274/tjps.galenos.2019.76094