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- Title
Recombinant versus natural human <sup>111</sup> In-β<sub>2</sub> -microglobulin for scintigraphic detection of Aβ<sub>2</sub> m amyloid in dialysis patients.
- Authors
Schäffer, Jürgen; Burchert, Wolfgang; Floege, Jürgen; Gielow, Peter; Kionka, Christine; Linke, Reinhold P.; Weiss, Elisabeth H.; Shaldon, Stanley; Koch, Karl M.
- Abstract
Recombinant versus natural human 111 In-β2 -microglobulin for scintigraphic detection of Aβ2 m amyloid in dialysis patients. Background. We previously introduced scintigraphy with 131 I-labeled β2 -microglobulin (β2 m), purified from uremic hemofiltrate, that is, “natural”β2 m, to specifically detect β2 m-associated amyloidosis (Aβ2 m) in hemodialysis (HD) patients. Methods. To improve the safety and resolution of the scan, we covalently bound the chelator diethylenetriaminepentaacetic acid to natural β2 m to allow radiolabeling with 111 In. In a second step, we generated and evaluated the usage of recombinant human β2 m (rhβ2 m) for scintigraphy. Results. Using natural 111 In-labeled β2 m, eight patients on HD for 0 to 17 years, without evidence of Aβ2 m, were scanned. Whole-body scintigraphy at 48 to 72 hours postinjection revealed no significant tracer accumulation over joint regions. In contrast, nine patients on HD for 10 to 21 years with clinical, radiological, or histologic (N = 4) evidence of Aβ2 m showed selective tracer uptake over various joint regions. Tracer accumulation in visceral organs, which could not be related to tracer elimination or metabolism, was not detected. Compared with the previous 131 I β2 m scan, scintigraphy with 111 In-labeled β2 m offered highly improved image contrast, increased sensitivity, and a 50 to 70% reduction of the radiation exposure. Scanning with 111 In-labeled recombinant human β2 m was performed in six patients: No significant tracer accumulation was observed over joint regions in two patients on short-term HD without evidence of Aβ2 m; in contrast, local...
- Subjects
RADIONUCLIDE imaging; DIALYSIS (Chemistry)
- Publication
Kidney International, 2000, Vol 58, Issue 2, p873
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1046/j.1523-1755.2000.00237.x