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- Title
Effect of Helicobacter pylori eradication on ongoing mutation of immunoglobulin genes in gastric MALT lymphoma.
- Authors
Fujimori, K.; Shimodaira, S.; Akamatsu, T.; Furihata, K.; Katsuyama, T.; Hosaka, S.
- Abstract
Gastric low-grade mucosa-associated lymphoid tissue (low-grade MALT) lymphomas has been associated with Helicobacter pylori (H. pylori) infection. Although infiltrating T cells with specificity for H. pylori are known to stimulate the development of MALT lymphomas, the effect of H. pylori eradication on rearranged immunoglobulin heavy chain (IgH) genes of low-grade gastric MALT lymphomas is unclear. Gastric biopsies from five cases were investigated by cloning and sequence analysis of rearranged IgH genes before and after the treatment for H. pylori. In all cases, IgH genes were mutated from their germline counterpart. The frequency of intraclonal sequence heterogeneity before the eradication of H. pylori varied from 0.25 to 0.49%. Clones obtained from the tumours before the eradication of H. pylori in cases 1 and 2 showed a tendency to display a mutation pattern by positive antigen selection and their monoclonarity disappeared after the eradication. The frequency of intraclonal sequence heterogeneity of the clones obtained from cases 3, 4 and 5 (0.12% in case 3, 0.10% in 4 and 0.18% in 5) after the eradication of H. pylori was lower than that in tumours before the eradication (0.30% in case 3, 0.49% in 4 and not determined in 5). These findings suggest that low-grade gastric MALT lymphomas expand due to the persistent presence of H. pylori in vivo. The characteristic feature of tumour clones obtained from the tumours after the eradication of H. pylori is a very low intraclonal heterogeneity, which may potentially be independent of H. pylori.
- Subjects
SPHINCTERS; LYMPHOMAS; TUMORS; HELICOBACTER; ENZYMES; HELICOBACTER pylori; GENES; CYSTS (Pathology); ANTIBIOTICS; STOMACH tumors; RESEARCH; GENETIC mutation; ANIMAL experimentation; RESEARCH methodology; B cell lymphoma; MEDICAL cooperation; EVALUATION research; DOCUMENTATION; NUCLEOTIDES; COMPARATIVE studies; AMINO acids; POLYMERASE chain reaction; HELICOBACTER diseases
- Publication
British Journal of Cancer, 2005, Vol 92, Issue 2, p312
- ISSN
0007-0920
- Publication type
journal article
- DOI
10.1038/sj.bjc.6602262