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- Title
Anion-Binding Properties of Short Linear Homopeptides.
- Authors
Modrušan, Matija; Glazer, Lucija; Otmačić, Lucija; Crnolatac, Ivo; Cindro, Nikola; Vidović, Nikolina; Piantanida, Ivo; Speranza, Giovanna; Horvat, Gordan; Tomišić, Vladislav
- Abstract
A comprehensive thermodynamic and structural study of the complexation affinities of tetra (L1), penta (L2), and hexaphenylalanine (L3) linear peptides towards several inorganic anions in acetonitrile (MeCN) and N,N-dimethylformamide (DMF) was carried out. The influence of the chain length on the complexation thermodynamics and structural changes upon anion binding are particularly addressed here. The complexation processes were characterized by means of spectrofluorimetric, 1H NMR, microcalorimetric, and circular dichroism spectroscopy titrations. The results indicate that all three peptides formed complexes of 1:1 stoichiometry with chloride, bromide, hydrogen sulfate, dihydrogen phosphate (DHP), and nitrate anions in acetonitrile and DMF. In the case of hydrogen sulfate and DHP, anion complexes of higher stoichiometries were observed as well, namely those with 1:2 and 2:1 (peptide:anion) complexes. Anion-induced peptide backbone structural changes were studied by molecular dynamic simulations. The anions interacted with backbone amide protons and one of the N-terminal amine protons through hydrogen bonding. Due to the anion binding, the main chain of the studied peptides changed its conformation from elongated to quasi-cyclic in all 1:1 complexes. The accomplishment of such a conformation is especially important for cyclopeptide synthesis in the head-to-tail macrocyclization step, since it is most suitable for ring closure. In addition, the studied peptides can act as versatile ionophores, facilitating transmembrane anion transport.
- Subjects
ION transport (Biology); SULFURIC acid; STABILITY constants; CIRCULAR dichroism; IONOPHORES; DIMETHYLFORMAMIDE; PHENYLALANINE
- Publication
International Journal of Molecular Sciences, 2024, Vol 25, Issue 10, p5235
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms25105235