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- Title
Noninvasive measure of microvascular nitric oxide function in humans using Very low frequency cutaneous laser Doppler flow spectra.
- Authors
Stewart, Julian Mark; Taneja, Indu; Medow, Marvin Scott; Goligorsky, Michael
- Abstract
While higher frequency oscillations (0.021-0.6 Hz) in cutaneous blood flow measured by laser Doppler flowmetry (LDF) relate to oscillations in blood pressure and sympathetic nerve activity, very low frequency oscillations (0.0095-0.021Hz, VLF) do not. We investigated whether VLF LDF power is nitric oxide (NO) specific. We used LDF combined with intradermal microdialysis in the calves of 22 healthy volunteers aged 19-27 years. LDF power spectral analysis was performed by windowed fast Fourier transform. We tested whether the NO synthesis inhibitor nitro-L-arginine (NLA) produced selective decreases in VLF power before and after stimulation with acetylcholine. NLA alone did not alter total power but selectively reduced VLF power by approximately 50%. LDF and spectral power increased markedly across all spectra with acetylcholine. This increase was blunted by NLA which selectively reduced VLF power by approximately 50%. The data suggest that VLF oscillations in the laser Doppler signal are NO dependent, increase with cholinergic stimulation, and have potential as a noninvasive marker for NO-dependent microvascular reactivity.
- Subjects
BLOOD flow measurement; LASER Doppler blood flowmetry; NITRIC oxide; FREQUENCIES of oscillating systems; ARGININE; ACETYLCHOLINE; CHOLINERGIC mechanisms
- Publication
FASEB Journal, 2007, Vol 21, Issue 6, pA1297
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fasebj.21.6.a1297-c