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- Title
Expression of 1α-Hydroxylase in Tissues Relevant to Energy Metabolism.
- Authors
Jia Li; Byrne, Mary E.; Donkin, Shawn S.; Buhman, Kimberly K.; Teegarden, Dorothy
- Abstract
High vitamin D and calcium intake is associated with reduced accumulation of body fat mass and insulin resistance. Expression of extra-renal 1a-hydroxylase (1OHase) has been reported in several tissues and contributes to local synthesis of 1a,25-dihydroxyvitamin D (1,25(OH)2D). The expression of 1OHase in tissues associated with energy metabolism, including adipose, liver, and muscle, has not been assessed. In the current study, the gene expression and dietary regulation of 1OHase was assessed by real time RT-PCR. Male Wistar rats were fed a high calcium (1.5%), high vitamin D (10,000 IU/kg), and dairy protein diet (20%) or a low calcium (0.25%), low vitamin D (400 IU/kg), and casein diet (20%) with either a high fat (40% energy) or high sucrose (60% energy) dietary background for 14 weeks. 1OHase expression was detected in liver, muscle, and adipose tissue. Expression did not differ with dietary level of calcium and vitamin D. However, 1OHase expression in liver was higher when the rats were fed a high fat diet compared to high sucrose diet (P=0.01). In conclusion, 1OHase was expressed in liver, muscle and adipose tissue, and its expression in the liver was regulated by dietary fat content. The data suggest diet type may influence local production of 1,25(OH)2D through changes in 1OHase expression and potentially impact tissue metabolism associated with fat mass and insulin resistance.
- Subjects
GENE expression; CHOLESTEROL hydroxylase; VITAMIN D; ENERGY metabolism; CALCIUM; LABORATORY rats
- Publication
FASEB Journal, 2007, Vol 21, Issue 6, pA1110
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fasebj.21.6.a1110-d