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- Title
Stimulation of arachidonic acid release by vasopressin in A7r5 vascular smooth muscle cells mediated by Ca<sup>2+</sup>-stimulated phospholipase A<sub>2</sub>
- Authors
Liu, Yingjie; Taylor, Colin W.
- Abstract
Abstract: Arachidonic acid (AA) regulates many aspects of vascular smooth muscle behaviour, but the mechanisms linking receptors to AA release are unclear. In A7r5 vascular smooth muscle cells pre-labelled with 3H-AA, vasopressin caused a concentration-dependent stimulation of 3H-AA release that required phospholipase C and an increase in cytosolic [Ca2+]. Ca2+ release from intracellular stores and Ca2+ entry via L-type channels or the capacitative Ca2+ entry pathway were each effective to varying degrees. Selective inhibitors of PLA2 inhibited the 3H-AA release evoked by vasopressin, though not the underlying Ca2+ signals, and established that cPLA2 mediates the release of AA. We conclude that in A7r5 cells vasopressin stimulates AA release via a Ca2+-dependent activation of cPLA2.
- Subjects
ARACHIDONIC acid; VASCULAR smooth muscle; PITUITARY hormones
- Publication
FEBS Letters, 2006, Vol 580, Issue 17, p4114
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2006.06.055