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- Title
Proteoglycan isolated from Phellinus linteus inhibits tumor growth through mechanisms leading to an activation of CD11c<sup>+</sup>CD8<sup>+</sup> DC and type I helper T cell-dominant immune state
- Authors
Kim, Gi-Young; Oh, Won-Kyo; Shin, Byung-Cheul; Shin, Yong-Il; Park, Young-Chul; Ahn, Soon-Cheol; Lee, Jae-Dong; Bae, Yoe-Sik; Kwak, Jong-Young; Park, Yeong-Min
- Abstract
Dendritic cells (DC) are known to not only induce the activation of T cells, but are also associated with the polarization of T cells. This study investigated whether or not proteoglycan (PG) isolated from Phellinus linteus induces the phenotypic and functional maturation of CD11c+ DC in vitro and in vivo. PG was found to induce the phenotypic and functional maturation of bone marrow-derived DC via Toll-like receptors (TLR) 2 and 4 in vitro. Administration of PG in vivo strongly inhibited the MCA-102 tumor growth and increase in vivo. The ratio of CD8+ DC to CD8- DC increased, and PG enhanced IL-12 and IFN-γ production, and expression of surface molecules including major histocompatibility complexes (MHC) classes I, MHC II, CD80, and CD86 in MCA-102-challenged mice. PG also caused a marked increase in the production of Th (helper T cells)-1 cytokine (IFN-γ) and a decrease in the production of Th-2 cytokine (IL-4) by splenic cells and inguinal lymph node cells in MCA-102 tumor-bearing mice. Furthermore, PG stimulated the proliferation of CD4+ and CD8+ T cells. In addition, a combination of PG and tumor lysate-pulsed DC inhibited completely the growth of MCA-102 cells in tumor-bearing mice. These results indicate that the administration of PG inhibited the tumor growth through a mechanism leading to a Th-1 dominant immune state and the activation of CD11c+CD8+ DC.
- Subjects
DENDRITIC cells; ANTIGEN presenting cells; LYMPHOCYTES; BONE marrow
- Publication
FEBS Letters, 2004, Vol 576, Issue 3, p391
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2004.09.047