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- Title
Expression and characterization of a cold-active and xylose-stimulated β-glucosidase from Marinomonas MWYL1 in Escherichia coli.
- Authors
Zhao, Wei; Peng, Rihe; Xiong, Aisheng; Fu, Xiaoyan; Tian, Yongsheng; Yao, Quanhong
- Abstract
The gene encoding a cold-active and xylose-stimulated β-glucosidase of Marinomonas MWYL1 was synthesized and expressed in Escherichia coli. The recombinant enzyme (reBglM1) was purified and characterized. The molecular mass of the purified reBglM1 determined by SDS-PAGE agree with the calculated values (50.6 Da). Optima of temperature and pH for enzyme activity were 40°C and 7.0, respectively. The enzyme exhibited about 20% activity at 5°C and was stable over the range of pH 5.5-10.0. The presence of xylose significantly enhanced enzyme activity even at higher concentrations up to 600 mM, with maximal stimulatory effect (about 1.45-fold) around 300 mM. The enzyme is active with both glucosides and galactosides and showed high catalytic efficiency ( k = 500.5 s) for oNPGlc. These characterizations enable the enzyme to be a promising candidate for industrial applications.
- Subjects
GENES; ESCHERICHIA coli; ENZYMES; GLYCOSIDASES; XYLOSE; TEMPERATURE
- Publication
Molecular Biology Reports, 2012, Vol 39, Issue 3, p2937
- ISSN
0301-4851
- Publication type
Article
- DOI
10.1007/s11033-011-1055-0