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- Title
Effects of intracellular Mn on the radiation resistance of the halophilic archaeon Halobacterium salinarum.
- Authors
Webb, Kimberly; Yu, Jerry; Robinson, Courtney; Noboru, Tomiya; Lee, Yuan; DiRuggiero, Jocelyne
- Abstract
Ionizing radiation (IR) is of particular interest in biology because its exposure results in severe oxidative stress to the cell's macromolecules. Our recent work with extremophiles supports the idea that IR resistance is most likely achieved by a metabolic route, effected by manganese (Mn) antioxidants. Biochemical analysis of 'super-IR resistant' mutants of H. salinarum, evolved over multiple cycles of exposure to high doses of IR, confirmed the key role for Mn antioxidants in the IR resistance of this organism. Analysis of the proteome of H. salinarum 'super-IR resistant' mutants revealed increased expression for proteins involved in energy metabolism, replenishing the cell with reducing equivalents depleted by the oxidative stress inflicted by IR. Maintenance of redox homeostasis was also activated by the over-expression of coenzyme biosynthesis pathways involved in redox reactions. We propose that in H. salinarum, increased tolerance to IR is a combination of metabolic regulatory adjustments and the accumulation of Mn-antioxidant complexes.
- Subjects
IONIZING radiation; MANGANESE isotopes; OXIDATIVE stress; HALOBACTERIUM salinarium; BACTERIAL evolution; BACTERIAL DNA metabolism; OXIDATION-reduction reaction; BACTERIA
- Publication
Extremophiles, 2013, Vol 17, Issue 3, p485
- ISSN
1431-0651
- Publication type
Article
- DOI
10.1007/s00792-013-0533-9