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- Title
Characterization of proteases in guts of Daphnia magna and their inhibition by Microcystis aeruginosa PCC 7806.
- Authors
Manish K. Agrawal; Anja Zitt; Divya Bagchi; Suvendra N. Bagchi; Eric von Elert
- Abstract
Many cyanobacteria produce peptides that inhibit mammalian proteases. The hypothesis that inhibitors of mammalian proteases produced by cyanobacteria also interfere with digestive proteases of natural cladoceran grazers was tested by comparing the effects of cyanobacterial protease inhibitors on digestive proteases from Daphnia magna and on commercially available bovine proteases. The major digestive proteases of D. magna are trypsins and chymotrypsins, which differ from those of bovine origin in substrate specificity and susceptibility to synthetic inhibitors. An extract from Microcystis aeruginosa strain PCC 7806 inhibited both types of D. magna proteases. Subsequent fractionation of the extract by high-performance liquid chromatography indicated that several inhibitors are produced by M. aeruginosa that differ in their specificity for the trypsins and chymotrypsins of D. magna. Two fractions differed in their inhibitory effect on proteases of D. magna and bovine origin; therefore, assessment of the impact of cyanobacterial protease inhibitors on natural communities requires the use of digestive proteases from ecologically relevant grazers. 2005 Wiley Periodicals, Inc. Environ Toxicol 20: 314322, 2005.
- Subjects
DAPHNIA magna; MICROCYSTIS; CHROOCOCCACEAE; PROTEASE inhibitors
- Publication
Environmental Toxicology, 2005, Vol 20, Issue 3, p314
- ISSN
1520-4081
- Publication type
Article
- DOI
10.1002/tox.20123