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- Title
Breathomics in Asthmatic Children Treated with Inhaled Corticosteroids.
- Authors
Ferraro, Valentina Agnese; Carraro, Silvia; Pirillo, Paola; Gucciardi, Antonina; Poloniato, Gabriele; Stocchero, Matteo; Giordano, Giuseppe; Zanconato, Stefania; Baraldi, Eugenio
- Abstract
Background: "breathomics" enables indirect analysis of metabolic patterns underlying a respiratory disease. In this study, we analyze exhaled breath condensate (EBC) in asthmatic children before (T0) and after (T1) a three-week course of inhaled beclomethasone dipropionate (BDP). Methods: we recruited steroid-naive asthmatic children for whom inhaled steroids were indicated and healthy children, evaluating asthma control, spirometry and EBC (in asthmatics at T0 and T1). A liquid-chromatography–mass-spectrometry untargeted analysis was applied to EBC and a mass spectrometry-based target analysis to urine samples. Results: metabolomic analysis discriminated asthmatic (n = 26) from healthy children (n = 16) at T0 and T1, discovering 108 and 65 features relevant for the discrimination, respectively. Searching metabolomics databases, seven putative biomarkers with a plausible role in asthma biochemical–metabolic processes were found. After BDP treatment, asthmatic children, in the face of an improved asthma control (p < 0.001) and lung function (p = 0.01), showed neither changes in EBC metabolomic profile nor in urinary endogenous steroid profile. Conclusions: "breathomics" can discriminate asthmatic from healthy children, with prostaglandin, fatty acid and glycerophospholipid as putative markers. The three-week course of BDP—in spite of a significant clinical improvement—was not associated with changes in EBC metabolic arrangement and urinary steroid profile.
- Subjects
BECLOMETHASONE dipropionate; ADRENOCORTICAL hormones; RESPIRATORY diseases; DATABASE searching; URINALYSIS; FATTY acids; METABOLOMICS
- Publication
Metabolites (2218-1989), 2020, Vol 10, Issue 10, p390
- ISSN
2218-1989
- Publication type
Article
- DOI
10.3390/metabo10100390