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- Title
Genome-wide Identification, Characterization, and Expression Analysis of PHT1 Phosphate Transporters in Wheat.
- Authors
Wan Teng; Yan-Yan Zhao; Xue-Qiang Zhao; Xue He; Wen-Ying Ma; Yan Deng; Xin-Ping Chen; Yi-Ping Tong
- Abstract
The PHT1 family of phosphate (Pi) transporters mediates phosphorus (P) uptake and re-mobilization in plants. A genome-wide sequence analysis of PHT1 genes in wheat (Triticum aestivum) was conducted, and their expression locations and responses to P availability were further investigated. We cloned 21 TaPHT1 genes from the homologous alleles at TaPHT1.1 to 1.10 through screening a BAC library and amplifying genomic sequences. The TaPHT1 transporters were clustered into five branches in the phylogenetic tree of PHT1 proteins, and the TaPHT1 genes from a given branch shared high similarities in sequences, expression locations, and responses to P availability. The seven tested PHT1 genes all showed Pi-transport activity in yeast (Saccharomyces cerevisiae) cells grown under both low Pi and high Pi conditions. The expression of TaPHT1.1/1.9, 1.2, and 1.10 were root specific. The expression of these TaPHT1 genes at flowering positively correlated with P uptake after stem elongation across three P application rates and two wheat varieties in a field experiment. Therefore, modification of PHT1 expression may improve P use efficiency in a broad regime of P availability.
- Subjects
WHEAT genetics; PHOSPHATE transport proteins; GENE expression in plants
- Publication
Frontiers in Plant Science, 2017, p1
- ISSN
1664-462X
- Publication type
Article
- DOI
10.3389/fpls.2017.00543