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- Title
A novel method for cell counting of Microcystis colonies in water resources using a digital imaging flow cytometer and microscope.
- Authors
Jungsu Park; Yongje Kim; Minjae Kim; Woo Hyoung Lee
- Abstract
Microcystis sp. is one of the most common harmful cyanobacteria that release toxic substances. Counting algal cells is often used for effective control of harmful algal blooms. However, Microcystis sp. is commonly observed as a colony, so counting individual cells is challenging, as it requires significant time and labor. It is urgent to develop an accurate, simple, and rapid method for counting algal cells for regulatory purposes, estimating the status of blooms, and practicing proper management of water resources. The flow cytometer and microscope (FlowCAM), which is a dynamic imaging particle analyzer, can provide a promising alternative for rapid and simple cell counting. However, there is no accurate method for counting individual cells within a Microcystis colony. Furthermore, cell counting based on two-dimensional images may yield inaccurate results and underestimate the number of algal cells in a colony. In this study, a three-dimensional cell counting approach using a novel model algorithm was developed for counting individual cells in a Microcystis colony using a FlowCAM. The developed model algorithm showed satisfactory performance for Microcystis sp. cell counting in water samples collected from two rivers, and can be used for algal management in fresh water systems.
- Subjects
MICROCYSTIS; CHROOCOCCACEAE; MICROCYSTINS; PHYTOPLANKTON; MICROBIAL peptides
- Publication
Environmental Engineering Research, 2019, Vol 24, Issue 3, p397
- ISSN
1226-1025
- Publication type
Article
- DOI
10.4491/eer.2018.266