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- Title
Genetic Diversity of Jordanian Wild Crocus Species as Detected by RAPD.
- Authors
Q. Syouf, Maha; Akash, Muhanad; Al-Gharaibeh, Moath; A. Shibli, Rida; Q. Alali, Feras
- Abstract
DNA among eight selected wild Crocus species was evaluated using Ra ndom Amplified Polymorphic DNA (RAPD). These are Crocus aleppicus Baker , C. cancellatus Herbert , C.cartwrightianus Herbert ,C. damascenus Herbert, C. hermoneus Ky.ex Maw subsp . palaestinus, C. hyemalis Boiss. & Blanche, C . moabiticus Bomm . & Dinsm . ex Bomm , and C. Pallasii supsp. Haussknechtii. The cultivated species; C. sativus and C. vernus were purchased from Italy . Genetic similarities between the Crocus species were evaluated by NTSYS-pc software using Dice coefficient. The dendogram was constructed using the Unweighted Pair Group Method (UPGMA). As a result, tested Crocus species were grouped into four well-separated monophyletic clusters. The cultivated species were clustered separately from the wild collected species. Wild Crocus species collected from different sites for the same species were clustered together. Cluste r analysis indicated a high degree of inter and intra species variation within the wild Crocus species. High genetic association was found among some wild species originated from the same collection site. The wild collected C. moabiticus (a Jordanian endemic species) was found to be very close to the cultivated C. sativus. The current results showed close relationship between C. sativus and C. moabiticus. This analysis demonstrated that RAPD-P CR has proved to be a successful tool to determine genetic diversity among studied Crocus species.
- Subjects
CROCUSES; PLANT species; PLANT genetics; RAPD technique; ENDEMIC plants
- Publication
Jordan Journal of Agricultural Sciences, 2016, Vol 12, Issue 3, p733
- ISSN
1815-8625
- Publication type
Article
- DOI
10.12816/0033375