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- Title
Urotensin II promotes the proliferation and secretion of vascular endothelial growth factor in rat dermal papilla cells by activating the Wnt-β-catenin signaling pathway.
- Authors
Congjuan Liao; Zhen Huang; Liuting Chen; Xiaorong Fan; Jun Peng; Xiaoqing Tan; Jianshe Yang; Xusheng Zhang
- Abstract
Introduction. Urotensin II (U II) is a kind of active peptide with a variety of biological effects, such as promoting cell proliferation and endocrine effects. The aim of this study is to investigate the effect of urotensin II on the proliferation and secretion of vascular endothelial growth factor (VEGF) in cultured rat dermal papilla cells (DPCs), and to explore its molecular mechanism. Materials and Methods. We used the DPCs isolated from the thoracic aortas of Wistar-Kyoto rats to run the CCK8 and ELISA assay, RC-PCR and Western blotting techniques to identify the effect of Urotensin II on the proliferation and secretion of VEGF in DPCs, data were analyzed by one-way ANOVA or t-test. Results. U II can increase the mRNA expression of proliferation markers Ki67 and PCNA. In addition, the Wnt/β-catenin pathway was activated by U II, but Wnt inhibitor DKK1 reversed the effect of U II. Conclusions. U II promoted the proliferation and secretion of VEGF in rat DPCs through activation of the Wnt-β-catenin signaling pathway.
- Subjects
CELLULAR signal transduction; SECRETION; RATS; ENDOCRINE glands; THORACIC aorta
- Publication
Italian Journal of Medicine, 2023, Vol 17, p1
- ISSN
1877-9344
- Publication type
Article
- DOI
10.4081/itjm.2023.1607