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- Title
Efficient Synthesis of Stearidonic Acid Enriched Triacylglycerol from Ahiflower Seed Oil via a Two- Step Enzyme Reaction.
- Authors
Changhwan Ju; Yu Jin Lee; Hui Su Yoon; Byung Hee Kim; In-Hwan Kim
- Abstract
Stearidonic acid (SDA) is a plant-based n-3 polyunsaturated fatty acid with multiple biological activities. The enrichment of SDA and synthesis of triacylglycerol (TAG) were carried out consecutively via two lipase-catalyzed reactions, hydrolysis, and esterification. First, SDA was enriched into a glyceride fraction from ahiflower seed oil by Candida rugosa lipase-catalyzed hydrolysis. Under the optimum conditions of 35℃, 0.1% lipase powder of Lipase OF, and 50% buffer solution (based on the weight of total substrate), SDA was enriched from 21.6 to 40.7 wt% in glyceride fraction. SDA-enriched TAG was then synthesized from the SDA-enriched glyceride and the SDA-enriched fatty acid via esterification using an inhouse immobilized lipase as a biocatalyst. The SDA-enriched fatty acid was obtained from part of the SDAenriched glyceride by saponification and the in-house immobilized lipase was prepared from Eversa® Transform 2.0 using Lewatit VP OC 1600 as a carrier. The optimum reaction conditions for the synthesis of TAG were a temperature of 50℃, an enzyme loading of 10%, and a vacuum of 10 mmHg. A maximum conversion to TAG of ca. 94% was achieved after 12 h under the optimum conditions.
- Subjects
LIPASES; OILSEEDS; OMEGA-3 fatty acids; UNSATURATED fatty acids; ENZYMES; FATTY acids
- Publication
Journal of Oleo Science, 2022, Vol 71, Issue 11, p1679
- ISSN
1345-8957
- Publication type
Article
- DOI
10.5650/jos.ess22215