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- Title
Fertilization success of the New Zealand geoduck, Panopea zelandica: Effects of sperm concentration, gamete age and contact time.
- Authors
Gribben, Paul E; Millar, Russell B; Jeffs, Andrew G
- Abstract
Valuable aquaculture and fisheries industries for the North American geoduck, Panopea generosa, have driven interest in developing similar ventures for other geoduck species including P. zelandica from New Zealand. However, little is known about the fertilization kinetics of this species, or the conditions under which the amount and quality of larvae can be maximized. We determined the effects of sperm concentration, gamete age and contact time on the fertilization success of P. zelandica using an extended-Vogel-Czihak-Chang-Wolf ( EVCCW) model. The model provided a good fit to laboratory data when applied to individual contact times. For a contact time of 10 s, optimal fertilization was achieved at concentrations of approximately 104 sperm μL−1, but this decreased to as little as 102 sperm μL−1 for contact times of several minutes. Optimal fertilization was always <100% (max. observed 70%) and the proportion of fertilized eggs decreased rapidly at sperm concentrations above the optimal. According to the model this was due to a slow block to polyspermy. If commercial hatchery facilities ensure that broodstock are in ripe condition, and use sperm <30-min old, then optimal fertilization can be expected at sperm densities of 102-103 sperm μL−1 at contact times of 5-10 min.
- Subjects
FERTILIZATION (Biology); PACIFIC geoduck; PANOPEA; SPERMATOZOA; GAMETES; AQUACULTURE
- Publication
Aquaculture Research, 2014, Vol 45, Issue 8, p1380
- ISSN
1355-557X
- Publication type
Article
- DOI
10.1111/are.12085