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- Title
Synergistic effects of mixed TiAlV and polyethylene wear particles on TNFa response in THP-1 macrophages.
- Authors
Baumann, Bernd; Rolf, Olaf; Jakob, Franz; Goebel, Sascha; Sterner, Thomas; Eulert, Jochen; Rader, Christof P.
- Abstract
TNFα is a potent osteoclastogenic cytokine that has a fundamental role in the pathogenesis of wear particleinduced osteolysis. Wear particles of one composition and their biological effects are well characterised. In contrast, little is known about the effects of mixed particles with respect to mix ratio and particle concentration. We evaluated the effects of different mix ratios of polyethylene and TiAlV particles on TNFα response. We used a human monocytic cell line (THP-1) in this in vitro study. THP-1 monocytes were differentiated to macrophage-like cells and exposed to different mixtures of lipopolysaccharide- detoxified polyethylene and TiAlV particles. TNFα was analysed in culture supernatants using ELISAs. Both polyethylene and TiAlV particles induced a dose- and time-related release of TNFα, with maximum levels after 6 h. A PE/TiAlV mix ratio of 36:1 at 108 particles/ ml induced significantly higher TNFα concentrations compared to equal particle concentrations of isolated TiAlV (ps0.047) or PE (ps0.044), indicating the synergistic effect of mixed particles. These results provide evidence that TiAlV and polyethylene particles have significant synergistic effects, depending on the mix ratio and particle concentrations. This supra-additive effect can contribute substantially to the pathogenesis of implant particle-induced osteolysis.
- Publication
Biomedical Engineering / Biomedizinische Technik, 2006, Vol 51, Issue 5/6, p360
- ISSN
0013-5585
- Publication type
Article
- DOI
10.1515/BMT.2006.070