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- Title
Periodontal Tissue Engineering After Tooth Replantation.
- Authors
da Silva Assunção, Luciana Reichert; Colenci, Renato; Ferreira do-Amaral, Caril Constante; Sonoda, Celso Koogi; Mogami Bomfim, Suely Regina; Okamoto, Roberta; Golim, Marjorie de Assis; Deffune, Elenice; Percinoto, Célio; Penha de Oliveira, Sandra Helena
- Abstract
Background: Blood-derived products, platelet-poor plasma (PPP) and platelet-rich plasma (PRP), constitute an approach in the enhancement of tissue healing. PRP has also been used as a scaffold for bone marrow stem cells in tissue engineering. This study evaluates the effect of PPP, calcium chloride--activated PRP (PRP/Ca), calcium chloride-and thrombin-activated PRP (PRP/Thr/Ca), and bone marrow mononuclear cells and PRP/Ca (BMMCs/PRP/Ca) on the healing of replanted dog teeth. Methods: After 30 minutes of extraction, teeth were replanted with 1) no material (control); 2) PPP; 3) PRP/Ca; 4) PRP/Thr/Ca; or 5) BMMCs/PRP/Ca. Histologic, histomorphometric, and immunohistochemical analysis was assessed 120 days after replantation. Data from histomorphometric analysis were analyzed statistically (analysis of variance, Tukey; P<0.05). Quantitative immunohistochemical analysis was analyzed by Kruskal-Wallis and Dunn post hoc test (P<0.05). Results: Flow cytometry analysis showed 55.98% of CD34+ and 32.67% of CD90/Thy-1 for BMMCs sample. BMMCs/PRP/Ca presented the largest areas of replacement resorption characterized by osseous ingrowth into cementum (P <0.05), with intense immunomarcation for tartrate-resistant acid phosphatase. The PRP/Ca group also showed areas of replacement resorption with significant immunomarcation for osteopontin. PRP/Thr/Ca presented no replacement resorption. PPP showed areas of inflammatory resorption, with immunomarcation for tartrate-resistant acid phosphatase. Conclusions: The results suggest that platelets activated with thrombin play an important role in the healing of tissues after tooth replantation. Additional studies are necessary to test other materials, because PRP/Ca did not present an appropriate scaffold for undifferentiated cells in the treatment of avulsed teeth.
- Subjects
BLOOD plasma; REGENERATION (Biology); STEM cells; TISSUE engineering; CALCIUM chloride; THROMBIN; LABORATORY dogs; TOOTH transplantation
- Publication
Journal of Periodontology, 2011, Vol 82, Issue 5, p758
- ISSN
0022-3492
- Publication type
Article
- DOI
10.1902/jop.2010.100448