We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Induction of epithelial-mesenchymal transition with O-glycosylated oncofetal fibronectin
- Authors
Ding, Yao; Gelfenbeyn, Kirill; Freire-de-Lima, Leonardo; Handa, Kazuko; Hakomori, Sen-itiroh
- Abstract
Abstract: Epithelial-mesenchymal transition (EMT) has been shown to play a key role in embryogenesis and cancer progression. We previously found that fibronectin (FN) carrying O-GalNAc at a specific site is selectively expressed in cancer and fetal cells/tissues, and termed oncofetal FN (onfFN). Here, we show that (i) a newly-established monoclonal antibody against FN lacking the O-GalNAc, termed normalFN (norFN), is useful for isolation of onfFN, (ii) onfFN, but not norFN, can induce EMT in human lung carcinoma cells, (iii) onfFN has a synergistic effect with transforming growth factor (TGF)β1 in EMT induction.
- Subjects
EPITHELIAL cells; EMBRYOLOGY; CANCER invasiveness; FIBRONECTINS; LUNG cancer; TRANSFORMING growth factors; CANCER cells
- Publication
FEBS Letters, 2012, Vol 586, Issue 13, p1813
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2012.05.020