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- Title
Parkin-co-regulated gene (PACRG) product interacts with tubulin and microtubules
- Authors
Ikeda, Takashi
- Abstract
Abstract: Parkin-co-regulated gene (PACRG) is a gene that shares a bidirectional promoter with Parkinson’s disease-related Parkin/Park2 gene. Recently, the PACRG gene product was implicated in the function of flagella. However, its exact function remains unknown. Here, I assessed the interaction between PACRG and tubulin. Co-sedimentation experiments revealed that PACRG directly binds to microtubules and α/β-tubulin heterodimers with high affinity. Microscopic studies showed that PACRG bundles microtubules and forms branched aggregates with unpolymerized tubulin dimers. The amino acid sequence of the microtubule-binding region of PACRG is highly conserved among various organisms, suggesting that tubulin binding is a basic property of PACRG. Structured summary: MINT-6439731, MINT-6439753, MINT-6439768: PACRG (genbank_protein_gi:169219363), tubulin alpha (uniprotkb:P02550), tubulin beta (uniprotkb:Q2XVP4) bind (MI:0407) by cosedimentation (MI:0027) MINT-6439796: PACRG (genbank_protein_gi:169219363), tubulin alpha (uniprotkb:P02550), tubulin beta (uniprotkb:Q2XVP4) bind (MI:0407) by pull-down (MI:0096)
- Subjects
TUBULINS; MICROTUBULES; DIMERS; OLIGOMERS
- Publication
FEBS Letters, 2008, Vol 582, Issue 10, p1413
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2008.02.081