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- Title
Characterization of a late gene, ORF67 from Bombyx mori nucleopolyhedrovirus
- Authors
Chen, Hui-qing; Chen, Ke-ping; Yao, Qin; Guo, Zhong-jian; Wang, Ling-ling
- Abstract
Abstract: Open reading frame 67 of Bombyx mori nucleopolyhedrovirus (BmORF67) is a homologue of Autographa californica multiple NPV ORF81. The gene is conserved among all baculoviruses and is thus considered a baculovirus core gene. The transcript of BmORF67 was detected at 18–72h post-infection (p.i.). Polyclonal antiserum raised to a His-BmORF67 fusion protein recognized BmORF67 in infected cell lysates from 24 to 72h p.i., suggesting that BmORF67 is a late gene. BmORF67 was not detected either in budded viruses or occlusion-derived virus. Immunofluoresence analysis showed that the protein located in the cytoplasm and interacted with host protein actin A3. In conclusion, BmORF67 is a late protein localized in the cytoplasm of infected cells that interacts with host protein.
- Subjects
SILKWORMS; BACULOVIRUSES; CYTOPLASM; IMMUNOFLUORESCENCE
- Publication
FEBS Letters, 2007, Vol 581, Issue 30, p5836
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2007.11.059