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- Title
The MlotiK1 channel transports ions along the canonical conduction pore
- Authors
Silverman, William R.; Heginbotham, Lise
- Abstract
Abstract: Although the cyclic nucleotide-modulated potassium channel from Mesorhizobium loti, MlotiK1, is easily studied using a 86Rb+ flux assay, its comparatively low activity raises serious concerns about the integrity of the purified protein. We investigated the pathway of uptake using a multi-pronged approach. First, we probed the conduction pathway using quaternary ammonium compounds known to block conduction in eukaryotic K+ channels. Second, we examined the effect of chemical modification of putative pore-lining residues. Our results are consistent with ions traversing MlotiK1 along a conduction pathway like that of the eukaryotic channels, but at a much slower rate.
- Subjects
POTASSIUM channels; PROTEINS; ION channels; AMMONIUM compounds
- Publication
FEBS Letters, 2007, Vol 581, Issue 26, p5024
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2007.09.017