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- Title
COMPARATIVE ANALYSIS OF HELICOBACTER PYLORI GENOTYPE IN NATURAL AND DRINKING WATERS OF ISPARTA AND ITS SURROUNDINGS WITH GASTRIC MUCOSA OF H. PYLORI INFECTED PATIENTS.
- Authors
Ekinci, Fatma Yeşim; Sofu, Aytül; Sağlam, Hidayet; Çakır, Onur; Arıkan, Mehmet Salih; Gören, İbrahim; Isler, Mehmet; Adiloğlu, Ali Kudret
- Abstract
Objective: There are studies that conclude that Helicobacter pylori is transmitted through fecal-oral and oral-oral routes. In this study, we aimed to determine the source and mode of transmission of H. pylori in H. pylori infected patients by comparing H. pylori species isolated both from drinking water samples and gastric mucosa samples obtained by endoscopy from people who had dyspeptic symptoms and had been using these sampled water supplies as drinking water by biochemical and genotypic analysis. Material and Method: Twelve drinking water samples from different points and 37 gastric mucosa samples obtained by endoscopy from patients were collected. 109 bp length DNA segments from 16S rRNA location ofH. pylori genome were amplified by nested PCR method from all water and human samples and the amplified genome were sequenced obtained from 7 of 12 water samples and 27 of 37 biopsy samples. The sequences were compared with the data from N1H by BLAST search program. Alignment has been performed by MEGA 4 alignment program between water and patient samples. Results: All the DNA sequences were detected to be related to the H. pylori genome with 92 to 98% similarity. With alignment, 85% to 100% matching were detected between patient and water supply samples of which all were already confirmed as H. pylori genome (92 to 98% similarity). Conclusion: These results suggest that contaminated and insufficiently disinfected drinking water supplies might be a reservoir for H. pylori which is a readily source for transmission to human in our region.
- Subjects
ISPARTA (Turkey); TURKEY; COMPARATIVE studies; HELICOBACTER pylori; HELICOBACTER pylori infections; GENETICS; GASTRIC mucosa; AQUATIC microbiology; INFECTIOUS disease transmission; GENE amplification; POLYMERASE chain reaction; NUCLEOTIDE sequence; RNA; PATIENTS
- Publication
Nobel Medicus Journal, 2011, Vol 7, Issue 2, p9
- ISSN
1305-2381
- Publication type
Article