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- Title
Identification of CD8[sup+] T Cell Epitopes in the Immediate Early 62 Protein (IE62) of Varicella-Zoster Virus, and Evaluation of Frequency of CD8 + T Cell Response to IE62, by Use of IE62 Peptides after Varicella Vaccination.
- Authors
Frey, Christian R.; Sharp, Margaret A.; Min, Andrew S.; Schmid, D. Scott; Loparev, Vladimir; Arvin, Ann M.
- Abstract
Varicella-zoster virus (VZV) causes varicella, establishes neuronal latency, and can reactivate, resulting in herpes zoster. VZV-specific T cells are important for controlling infection. VZV immediate early protein 62 (IE62) is recognized by cytotoxic T cells from immune individuals, but no CD8[SUP+] T cell epitopes have been defined for any VZV protein. CD8[SUP+] T cell frequencies were assessed by cytokine flow cytometry (CFC), by use of synthetic-peptide pools corresponding to the IE62 sequence. IE62 peptide-specific CD8[SUP+] T cells were below the threshold of detection, by direct CFC of either whole blood or peripheral blood mononuclear cells (PBMCs). Activated CD8[SUP+]CD69[SUP+] T cells that produced interferon-γ were detectable after in vitro restimulation of PBMCs, and restricted epitopes were identified for HLA-A*0201-positive subjects. Varicella vaccination of 3 VZV-immune subjects was associated with increases in IE62 peptide-specific CD8[SUP+] T cells, a finding indicating that in vivo re-exposure boosts memory immunity to this important viral protein.
- Subjects
VARICELLA-zoster virus; T cells; EPITOPES; IMMUNIZATION
- Publication
Journal of Infectious Diseases, 2003, Vol 188, Issue 1, p40
- ISSN
0022-1899
- Publication type
Article
- DOI
10.1086/375828