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- Title
Milk whey-specific immune complexes in allergic and non-allergic subjects.
- Authors
Bell, S. J. D.; Potter, P. C.
- Abstract
We developed an antigen- and isotype-specific ELISA for the rapid detection of native serum immune complexes (IC). It is a sandwich assay, in which a solid phase antigenspecific capture antibody selectively binds the antigen specific IC via the antigen. The isotype of the bound IC is then identified using an enzyme-labelled indicator antibody. Using this sensitive assay system we were able to detect native serum milk whey-specific immune complexes (SM IC) of the IgG, IgE arid IgA isotypes. Detectable amounts of native serum SMIC of all three isotypes were found in sera of the majority of both atopic and non-atopic subjects. The ELISA was then used to compare the levels of native IgE SMIC in sea of milk PAST positive atopic patients with those found in milk PAST negative atopic patient sera. Milk RAST positive patient sera were found to have significantly higher mean levels (<em>P</em>≥ 0.005) of native IgE SMIC than milk RAST negative sera. Sera of other atopic individuals were also found to contain significantly higher mean levels (<em>P</em>≥0.005) of native IgG and IgA SMIC than non-atopic donors. IgE IC may specifically be involved in adverse symptoms seen in milk allergic patients.
- Subjects
MILK; ALLERGIES; PATIENTS; IMMUNE complexes; ANTIGENS; SERUM
- Publication
Allergy, 1988, Vol 43, Issue 7, p497
- ISSN
0105-4538
- Publication type
Article
- DOI
10.1111/j.1398-9995.1988.tb01626.x