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- Title
Successful use of artificial insemination in the production of red-legged partridges ( Alectoris rufa).
- Authors
Abouelezz, F. M. K.; Castaño, C.; Toledano-Díaz, A.; Esteso, M. C.; López-Sebastián, A.; Dávila, S. G.; Gil, M. G.; Cuenca, O. Torres; Campo, J. L.; Blesbois, E.; Santiago-Moreno, J.
- Abstract
This paper reports the first successful artificial insemination (AI) of red-legged partridges ( Alectoris rufa), the fertility rate achieved, and the length of time sperm cells can survive inside the oviduct (i.e., the post-AI fertile period). Semen from 20 mature males was collected by massage and pooled for use in single intravaginal inseminations (20 μL of fresh, undiluted semen) of eight females (15 × 10 sperm/female). The latter's eggs were then collected for 4 weeks, and the fertilizing capacity of the sperm used in the preceding AIs was determined by observing the development of the blastoderm. The duration of the post-AI fertile period was determined by subjecting fertilized eggs to the SP-holes assay. A second experiment was then performed to measure the percentage of viable embryos at 20 days of incubation (30 %) and hatchability (40 %). The mean fertility rate was 34.5 ± 11.7 % and the SP-holes value 17.3 ± 4.3. The mean duration of the post-AI fertile period was 3 weeks. In conclusion, the present work reports the first ever birth of partridge chicks following AI and shows that this procedure may be of use in the conservation of this species.
- Subjects
ARTIFICIAL insemination; FERTILITY; RED-legged partridge; SPERMATOZOA; ALECTORIS
- Publication
European Journal of Wildlife Research, 2015, Vol 61, Issue 4, p645
- ISSN
1612-4642
- Publication type
Article
- DOI
10.1007/s10344-015-0929-1