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- Title
Platelet-Rich Fibrin Constructs Elute Higher Concentrations of Transforming Growth Factor-β1 and Increase Tendon Cell Proliferation Over Time when Compared to Blood Clots: A Comparative In Vitro Analysis.
- Authors
Visser, Lance C.; Arnoczky, Steven P.; Caballero, Oscar; Egerbacher, Monika
- Abstract
Objective: To compare the concentration of a representative growth factor (transforming growth factor-beta [TGF-β]1) eluted from a platelet-rich fibrin matrix (PRFMatrix), a platelet-rich fibrin membrane (PRFMembrane), and a whole blood clot (BC) over time, and to compare the mitogenic effect of the eluents from each construct. Study Design: In vitro study. Sample Population: PRFMatrix, PRFMembrane, and BC (n=4/construct/time point). Methods: Each construct was placed in tissue culture wells containing media for 7 days. The media was collected and replenished on days 1, 3, 5, and 7 and the concentration of eluted TGF-β1 was measured by enzyme-linked immunosorbent assay. Canine tendon cells were subjected to additional aliquots of the conditioned media and the amount of cell proliferation compared. Results: The media from both PRFM (PRFMatrix and PRFMembrane) constructs contained significantly more ( P≤.026) TGF-β1 at days 1 and 3 and produced a significant increase ( P≤.044) in cell proliferation at all time points compared with the BC. The PRFMembrane media contained significantly more ( P≤.05) TGF-β1 at days 1 and 3 and produced a significant increase ( P≤.002) in cell proliferation at all time points compared with the PRFMatrix. Conclusions: Both PRFM constructs are comprised of a dense fibrin scaffold that contains increased concentrations of TGF-β1 and are capable of increasing tendon cell proliferation over time when compared with a BC. Clinical Relevance: The sustained increase in growth factor availability in PRFM constructs may be beneficial in the healing of biologically compromised tissues.
- Publication
Veterinary Surgery, 2010, Vol 39, Issue 7, p811
- ISSN
0161-3499
- Publication type
Article
- DOI
10.1111/j.1532-950X.2010.00739.x