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- Title
A protease/peptidase from culture medium of Flammulina velutipes that acts on arabinogalactan-protein.
- Authors
Yoshimi, Yoshihisa; Sugawara, Yumi; Hori, Chiaki; Igarashi, Kiyohiko; Kaneko, Satoshi; Tsumuraya, Yoichi; Kotake, Toshihisa
- Abstract
Arabinogalactan-proteins (AGPs) are highly diverse plant proteoglycans found on the plant cell surface. AGPs have large arabinogalactan (AG) moieties attached to a core-protein rich in hydroxyproline (Hyp). The AG undergoes hydrolysis by various glycoside hydrolases, most of which have been identified, whereas the core-proteins is presumably degraded by unknown proteases/peptidases secreted from fungi and bacteria in nature. Although several enzymes hydrolyzing other Hyp-rich proteins are known, the enzymes acting on the core-proteins of AGPs remain to be identified. The present study describes the detection of protease/peptidase activity toward AGP core-proteins in the culture medium of winter mushroom (Flammulina velutipes) and partial purification of the enzyme by several conventional chromatography steps. The enzyme showed higher activity toward Hyp residues than toward proline and alanine residues and acted on core-proteins prepared from gum arabic. Since the activity was inhibited in the presence of Pefabloc SC, the enzyme is probably a serine protease. The degradation of the core-protein of AGPs by a protease/peptidase from winter mushroom.
- Subjects
PEPTIDASE; ARABINOGALACTAN; FLAMMULINA velutipes
- Publication
Bioscience, Biotechnology & Biochemistry, 2017, Vol 81, Issue 3, p475
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2016.1258985