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- Title
Inhibitory effects of caffeine on gustatory plasticity in the nematode Caenorhabditis elegans.
- Authors
Urushihata, Takuya; Takuwa, Hiroyuki; Higuchi, Yukako; Sakata, Kazumi; Wakabayashi, Tokumitsu; Nishino, Asuka; Matsuura, Tetsuya
- Abstract
The effects of caffeine on salt chemotaxis learning were investigated using the nematodeCaenorhabditis elegans. To estimate the degree of salt chemotaxis learning, nematodes were placed in a mixed solution of NaCl and caffeine, and then the chemotaxis index of NaCl was obtained from the nematodes placed on agar medium after pre-exposure to caffeine concentrations of 0.01, 0.1, 0.3, and 1.0%. Locomotor activity and preference behavior for caffeine were also estimated under these caffeine conditions. Nematodes pre-exposed to 0.3% caffeine showed inhibition of salt chemotaxis learning. Additional experiments indicated that nematodes showed a preference response to the middle concentration of caffeine (0.1%), with preference behavior declining in the 0.3% caffeine condition. Stable locomotor activity was observed under 0.01–0.3% caffeine conditions. These results suggest that salt chemotaxis learning with 0.3% caffeine is useful for investigating the effects of caffeine on learning in nematodes. We developed the nematode as a model animal for studying the effects of caffeine on learning. Nematodes pre-exposed to 0.3% caffeine showed inhibition of learning.
- Subjects
PHYSIOLOGICAL effects of caffeine; CAENORHABDITIS elegans; CHEMOTAXIS; PHYSIOLOGY
- Publication
Bioscience, Biotechnology & Biochemistry, 2016, Vol 80, Issue 10, p1990
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2016.1191327