We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Detection of the tumour suppressor gene TP53 and expression of p53, Bcl-2 and p63 proteins in canine transmissible venereal tumour.
- Authors
Stockmann, D.; Ferrari, H. F.; Andrade, A. L.; Cardoso, T. C.; Luvizotto, M. C. R.
- Abstract
Canine transmissible venereal tumour (CTVT) is a neoplasm transmitted among healthy dogs by direct contact with injured skin and/or mucous tissue. This study aimed to identify the TP53 gene, messenger RNA (mRNA) as well as the expression of p53, Bcl-2 and p63 proteins in histological sections of 13 CTVT samples at different stages of evolution. The in situ hybridization (ISH) and in situ reverse transcriptase polymerase chain reaction (RT-PCR) assays were used, which showed the DNA homologous to TP53 and its respective mRNA in 92.3% of the samples. We detected p53, p63 and Bcl-2 proteins in most of the cell samples in different grades of intensity. In addition, 46% of the samples were in the progressive and 54% in the regression phase. This is the first description of these proteins and a detailed study of their role in CTVT cells needs to be addressed in or to verify how these cells undergo apoptosis.
- Subjects
GENE expression; DOG diseases; ANIMAL diseases; POLYMERASE chain reaction; VETERINARY genetics; GENETICS
- Publication
Veterinary & Comparative Oncology, 2011, Vol 9, Issue 4, p251
- ISSN
1476-5810
- Publication type
Article
- DOI
10.1111/j.1476-5829.2010.00260.x