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- Title
Identification of small molecules inducing apoptosis by cell-based assay using fission yeast deletion mutants.
- Authors
Kyung-Sook Chung; Seung-Hee Lee; Shin-Jung Choi; Kyung-Sun Hur; Kwang-Lae Hoe; Dong-Uk Kim; Sondra Goehle; Hyung-Bae Kim; Kyung-Bin Song; Hyang-Sook Yoo; Ki-Hwan Bae; Julian Simon; Misun Won
- Abstract
Summary The cell-based assay using yeast deletion mutants has been recognized as an efficient analysis to discover therapeutic compounds and reveal their mode of action. In this study, S. pombe deletion mutants-based HTS screening was carried out to identify potential anti-cancer agents. The NCI chemical library of 5700 compounds was screened using kit strains, which consisted of S. pombe mutants harboring deletions in genes involved in DNA repair and mitotic control. During the screening, we identified 40 compounds conferring growth inhibition of S. pombe. Their anti-tumorigenic properties were examined by phenotypic effect on S. pombe, flow cytometry and apoptosis analysis of human cancer. Here, we report hit compounds inducing apoptosis for development of anti-cancer agents suggesting that S. pombe deletion mutants are useful in identifying potential anti-cancer agents in human cancer therapeutics.
- Subjects
APOPTOSIS; MICROBIOLOGICAL assay; SCHIZOSACCHAROMYCES; PHENOTYPES
- Publication
Investigational New Drugs, 2008, Vol 26, Issue 4, p299
- ISSN
0167-6997
- Publication type
Article
- DOI
10.1007/s10637-007-9100-5