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- Title
Antioxidative role of selenoprotein W in oxidant -induced chicken splenic lymphocyte death.
- Authors
Yu, Dong; Zhang, Zi-wei; Yao, Hai-dong; Li, Shu; Xu, Shi-wen
- Abstract
To verify the antioxidative role of SelW in oxidant-induced chicken splenic lymphocyte, in this report, the influence of selenite supplementation and SelW gene silence on HO-mediated cell viability and cell apoptosis in cultured splenic lymphocyte derived from spleen of chicken were examined. The cultured cells were treated with sodium selenite and HO, or knocked down SelW with small interfering RNAs (siRNAs). The lymphocytes were examined for cell viability, cell apoptosis and mRNA expression levels of SelW and apoptosis-related genes (Bcl-2, Bax, Bak-1, caspase-3 and p53). The results show that the mRNA expression of SelW were effectively increased after treatment with sodium selenite, and HO-induced cell apoptosis was significantly decreased and cell viability was significantly increased. 20 μM HO was found to induce cell apoptosis and decrease cell viability, which was alleviated obviously when cells were pretreated with sodium selenite before exposure to 20 μM HO. Meanwhile, HO induced a significantly up-regulation of the Bax/Bcl-2 ratio, Bax, Bak-1, caspase-3 and p53 and down-regulation of Bcl-2 ( P < 0.05). When lymphocytes were pretreated with Se before treated with HO, the Bax/Bcl-2 ratio and mRNA expression of those genes were significantly decreased, and Bcl-2 was increased ( P < 0.05). SelW siRNA-transfected cells were more sensitive to the oxidative stress induced by treatment of HO than control cells. Silencing of the lymphocyte SelW gene decreased their cell viability, and increased their apoptosis rate and susceptibility to HO. Silencing of SelW significantly up-regulated the Bax/Bcl-2 ratio, Bax, Bak-1, caspase-3 and p53 and down-regulated Bcl-2 ( P < 0.05). The present study demonstrates that SelW plays an important role in protection of splenic lymphocyte of birds from oxidative stress.
- Subjects
SELENOPROTEINS; LYMPHOCYTES; MESSENGER RNA; APOPTOSIS; CASPASES; SMALL interfering RNA
- Publication
BioMetals, 2014, Vol 27, Issue 2, p277
- ISSN
0966-0844
- Publication type
Article
- DOI
10.1007/s10534-014-9708-9