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- Title
Arabidopsis transcriptome responses to low water potential using highthroughput plate assays.
- Authors
Gonzalez, Stephen; Swift, Joseph; Yaaran, Adi; Jiaying Xu; Miller, Charlotte; Illouz-Eliaz, Natanella; Nery, Joseph R.; Busch, Wolfgang; Zait, Yotam; Ecker, Joseph R.
- Abstract
Soil-free assays that induce water stress are routinely used to investigate drought responses in the plant Arabidopsis thaliana. Due to their ease of use, the research community often relies on polyethylene glycol (PEG), mannitol, and salt (NaCl) treatments to reduce the water potential of agar media, and thus induce drought conditions in the laboratory. However, while these types of stress can create phenotypes that resemble those of water deficit experienced by soilgrown plants, it remains unclear how these treatments compare at the transcriptional level. Here, we demonstrate that these different methods of lowering water potential elicit both shared and distinct transcriptional responses in Arabidopsis shoot and root tissue. When we compared these transcriptional responses to those found in Arabidopsis roots subject to vermiculite drying, we discovered many genes induced by vermiculite drying were repressed by low water potential treatments on agar plates (and vice versa). Additionally, we also tested another method for lowering water potential of agar media. By increasing the nutrient content and tensile strength of agar, we show the ‘hard agar’ (HA) treatment can be leveraged as a high-throughput assay to investigate natural variation in Arabidopsis growth responses to low water potential.
- Subjects
MANNITOL; WATER use; ARABIDOPSIS; AGAR; POLYETHYLENE glycol; TRANSCRIPTOMES; WATER purification; ARABIDOPSIS thaliana
- Publication
eLife, 2024, p1
- ISSN
2050-084X
- Publication type
Article
- DOI
10.7554/eLife.84747