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- Title
Imaging Flow Cytometry and Convolutional Neural Network-Based Classification Enable Discrimination of Hematopoietic and Leukemic Stem Cells in Acute Myeloid Leukemia.
- Authors
Hybel, Trine Engelbrecht; Jensen, Sofie Hesselberg; Rodrigues, Matthew A.; Hybel, Thomas Engelbrecht; Pedersen, Maya Nautrup; Qvick, Signe Håkansson; Enemark, Marie Hairing; Bill, Marie; Rosenberg, Carina Agerbo; Ludvigsen, Maja
- Abstract
Acute myeloid leukemia (AML) is a heterogenous blood cancer with a dismal prognosis. It emanates from leukemic stem cells (LSCs) arising from the genetic transformation of hematopoietic stem cells (HSCs). LSCs hold prognostic value, but their molecular and immunophenotypic heterogeneity poses challenges: there is no single marker for identifying all LSCs across AML samples. We hypothesized that imaging flow cytometry (IFC) paired with artificial intelligence-driven image analysis could visually distinguish LSCs from HSCs based solely on morphology. Initially, a seven-color IFC panel was employed to immunophenotypically identify LSCs and HSCs in bone marrow samples from five AML patients and ten healthy donors, respectively. Next, we developed convolutional neural network (CNN) models for HSC-LSC discrimination using brightfield (BF), side scatter (SSC), and DNA images. Classification using only BF images achieved 86.96% accuracy, indicating significant morphological differences. Accuracy increased to 93.42% when combining BF with DNA images, highlighting differences in nuclear morphology, although DNA images alone were inadequate for accurate HSC-LSC discrimination. Model development using SSC images revealed minor granularity differences. Performance metrics varied substantially between AML patients, indicating considerable morphologic variations among LSCs. Overall, we demonstrate proof-of-concept results for accurate CNN-based HSC-LSC differentiation, instigating the development of a novel technique within AML monitoring.
- Subjects
HEMATOPOIETIC stem cells; ACUTE myeloid leukemia; CONVOLUTIONAL neural networks; MYELOID cells; FLOW cytometry; BONE marrow; PAIRED comparisons (Mathematics)
- Publication
International Journal of Molecular Sciences, 2024, Vol 25, Issue 12, p6465
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms25126465