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- Title
Diagnostic performance of metagenomic next-generation sequencing in non-tuberculous mycobacterial pulmonary disease when applied to clinical practice.
- Authors
Wei, Wei; Cao, Jie; Wu, Xiao-cui; Cheng, Li-ping; Shen, Xiao-na; Sha, Wei; Sun, Qin
- Abstract
Objective: To compare non-tuberculous mycobacterial pulmonary disease (NTMPD) diagnosis by metagenomic next-generation sequencing (mNGS) with Bactec mycobacterial growth indicator tube (MGIT) 960. Methods: A total of 422 patients with suspected NTMPD in Shanghai Pulmonary Hospital between January 2020 and May 2021 were retrospectively analyzed; 194 were diagnosed with NTMPD. The diagnostic performance of mNGS and MGIT 960 for NTMPD was assessed. Receiver operating characteristic (ROC) curves and areas under curve (AUCs) were compared. Results: The sensitivity of mNGS in NTMPD diagnosis was 81.4% and higher than that of MGIT 960 (53.6%). The specificity of mNGS in NTMPD diagnosis was 97.8%, similar to that of MGIT 960 (100%). The sensitivity of combined mNGS and MGIT 960 in NTMPD diagnosis was 91.8%. The sensitivity of mNGS for bronchoalveolar lavage fluid (BALF), pulmonary puncture tissue fluid, and sputum was 84.8%, 80.6%, and 77.5%, respectively; all were higher than that of MGIT 960 (P < 0.05). The AUC of mNGS and MGIT 960 was 0.897 and 0.768, respectively. The AUC of mNGS were BALF (0.916), pulmonary puncture tissue fluid (0.903), and sputum (0.870). Conclusion: The sensitivity of mNGS was superior to that of Bactec MGIT 960; the specificity in NTMPD diagnosis was similar. mNGS shows effective performance in NTMPD diagnosis.
- Subjects
CHINA; MYCOBACTERIAL disease diagnosis; LUNG disease diagnosis; BODY fluid analysis; SEQUENCE analysis; BRONCHOALVEOLAR lavage; SPUTUM; RETROSPECTIVE studies; PHARMACOKINETICS; COMPARATIVE studies; GENOMICS; DESCRIPTIVE statistics; RESEARCH funding; RECEIVER operating characteristic curves; SENSITIVITY &; specificity (Statistics); OLIGONUCLEOTIDE arrays; MICROBIAL sensitivity tests
- Publication
Infection, 2023, Vol 51, Issue 2, p397
- ISSN
0300-8126
- Publication type
Article
- DOI
10.1007/s15010-022-01890-z