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- Title
Fluorescence molecular tomography of DiR-labeled mesenchymal stem cell implants for osteochondral defect repair in rabbit knees.
- Authors
Berninger, Markus; Mohajerani, Pouyan; Kimm, Melanie; Masius, Stephan; Ma, Xiaopeng; Wildgruber, Moritz; Haller, Bernhard; Anton, Martina; Imhoff, Andreas; Ntziachristos, Vasilis; Henning, Tobias; Meier, Reinhard; Berninger, Markus T; Imhoff, Andreas B; Henning, Tobias D
- Abstract
<bold>Objectives: </bold>To assess labelling efficiency of rabbit mesenchymal stem cells (MSCs) using the near-infrared dye 1,1'-dioctadecyl-3,3,3',3'-tetramethylindotricarbocyanine iodide (DiR) and detection of labelled MSCs for osteochondral defect repair in a rabbit model using fluorescence molecular tomography-X-ray computed tomography (FMT-XCT).<bold>Methods: </bold>MSCs were isolated from New Zealand White rabbits and labelled with DiR (1.25-20 μg/mL). Viability and induction of apoptosis were assessed by XTT- and Caspase-3/-7-testing. Chondrogenic potential was evaluated by measurement of glycosaminoglycans. Labelled cells and unlabeled controls (n = 3) underwent FMT-XCT imaging before and after chondrogenic differentiation. Osteochondral defects were created surgically in rabbit knees (n = 6). Unlabeled and labelled MSCs were implanted in fibrin-clots and imaged by FMT-XCT. Statistical analyses were performed using multiple regression models.<bold>Results: </bold>DiR-labelling of MSCs resulted in a dose-dependent fluorescence signal on planar images in trans-illumination mode. No significant reduction in viability or induction of apoptosis was detected at concentrations below 10 μg DiR/mL (p > .05); the chondrogenic potential of MSCs was not affected (p > .05). FMT-XCT of labelled MSCs in osteochondral defects showed a significant signal of the transplant (p < .05) with additional high-resolution anatomical information about its osteochondral integration.<bold>Conclusions: </bold>FMT-XCT allows for detection of stem cell implantation within osteochondral regeneration processes.<bold>Key Points: </bold>• DiR-labelling of MSCs shows no toxic side effects or impairment of chondrogenesis. • Fluorescence molecular tomography allows for detection of MSCs for osteochondral defect repair. • FMT-XCT helps to improve evaluation of cell implantation and osteochondral regeneration processes.
- Subjects
FLUORESCENCE microscopy; MESENCHYMAL stem cells; COMPUTED tomography; LABORATORY rabbits; STEM cell transplantation
- Publication
European Radiology, 2017, Vol 27, Issue 3, p1105
- ISSN
0938-7994
- Publication type
journal article
- DOI
10.1007/s00330-016-4457-5