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- Title
Novel virus discovery in field-collected mosquito larvae using an improved system for rapid determination of viral RNA sequences (RDV ver4.0).
- Authors
Yamao, Takuya; Eshita, Yuki; Kihara, Yuki; Satho, Tomomitsu; Kuroda, Makoto; Sekizuka, Tsuyoshi; Nishimura, Miho; Sakai, Kouji; Watanabe, Shumpei; Akashi, Hiroomi; Yupha Rongsriyam; Narumon Komalamisra; Raweewan Srisawat; Miyata, Takeshi; Sakata, Akira; Hosokawa, Masato; Nakashima, Manabu; Kashige, Nobuhiro; Miake, Fumio; Fukushi, Shuetsu
- Abstract
In this study, we improved a method for rapid determination of viral RNA sequences (RDV) to overcome the limitations of previous versions. The RDV ver4.0 method can detect RNA sequences with at least 1,000 copies as starting material. A novel virus, which was isolated from field-collected Aedes aegypti larvae in the Phasi Charoen district of Thailand using C6/36 cells, was identified using the RDV ver4.0 protocol. The virus was named Phasi Charoen virus (PhaV). We used a high-throughput pyrosequencing approach to obtain more information about the genome sequence of PhaV. Analysis of a phylogenic tree based on amino acid sequences strongly suggested that PhaV belongs to the family Bunyaviridae.
- Subjects
MOSQUITO vectors; RNA viruses; AEDES aegypti; BUNYAVIRUSES; PATHOGENIC microorganisms
- Publication
Archives of Virology, 2009, Vol 154, Issue 1, p153
- ISSN
0304-8608
- Publication type
Report
- DOI
10.1007/s00705-008-0285-5