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- Title
An element downstream of the transcription start site is required for activation of Bombyx mori nucleopolyhedrovirus bro-c promoter.
- Authors
Suzuki, M. G.; Kang, W.-K.; Maeda, S.
- Abstract
Summary. Bombyx mori nucleopolyhedrovirus (BmNPV) contains five baculovirus repeated ORF (bro) genes. We have previously reported that all of these genes (bro-a, b, c, d and e) are transcribed as early genes and require viral factor(s) for their expression. In this study, we investigated the mechanism of promoter activation of the bro-c gene. Transient expression assays using genomic libraries of BmNPV indicated that the baculoviral trans-regulator IE-1 is responsible for activating the bro-c promoter. To identify essential site(s) for promoter activation, mutations were introduced to the promoter region of bro-c. Interestingly, it was shown that the pentanucleotide sequence CACGC located 30 nucleotides downstream of the RNA start site was essential for bro-c promoter activation. In addition, the RNA start site and the spacing between the RNA start site and CACGC were also required for promoter activation. By introducing a CACGC sequence into the corresponding region of the bro-b promoter, which is not normally trans-activated by IE-1, we demonstrated that this pentanucleotide motif has the ability to confer trans-activation by IE-1 on a promoter. Gel retardation experiments also showed a sequence-specific DNA binding protein induced by baculovirus infection interacts with the CACGC motif.
- Subjects
SILKWORMS; BACULOVIRUS genetics; GENETIC transcription; VIRAL genetics; PROMOTERS (Genetics); DNA-binding proteins
- Publication
Archives of Virology, 2001, Vol 146, Issue 3, p495
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s007050170158