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- Title
Induction of NO synthase 2 in ventricular cardiomyocytes incubated with a conventional bicarbonate dialysis bath.
- Authors
Eleonora Grandi; Marco Govoni; Simone Furini; Stefano Severi; Emanuele Giordano; Antonio Santoro; Silvio Cavalcanti
- Abstract
Background. In hypotension-prone patients, conventional bicarbonate dialysis (BD) causes a reduced cardiovascular tolerance to the treatment with respect to acetate-free biofiltration (AFB). One possible explanation is an overproduction of endogenous NO (nitric oxide) due to the residual quote of acetate (4 mM) in the BD dialysate formulation. NO overload might cause the impairment of cardiovascular reactivity observed during BD. In this study, a potential direct impact of the residual quote of acetate on the cardiac cells was investigated. Methods. Ventricular cardiac myocytes isolated from adult rat hearts were treated with three different dialysis baths with or without acetate: BD, AFB and AFB + 4 mM of acetate (AFB+). Corresponding levels of expression of the inducible NO synthase 2 (NOS2) were assessed after the treatments along with the measurement of single-cell action potential (AP). Results. Incubation with acetate-containing dialysis solutions significantly enhanced (P P Conclusion. These findings show that the residual quote of acetate of the BD bath formulation affects the expression of NOS2 and the duration of AP in cardiac cells. This might cause the cardiac contractile impairment in unstable patients during BD treatment.
- Subjects
NITROGEN oxides; HEART cells; DIALYSIS (Chemistry); NITRIC-oxide synthases
- Publication
Nephrology Dialysis Transplantation, 2008, Vol 23, Issue 7, p2192
- ISSN
0931-0509
- Publication type
Article
- DOI
10.1093/ndt/gfn020