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- Title
Asiatic Acid Inhibits Adipogenic Differentiation of Bone Marrow Stromal Cells.
- Authors
Li, Zheng-Wei; Piao, Cheng-dong; Sun, Hong-Hui; Ren, Xian-Sheng; Bai, Yun-Shen
- Abstract
Bone marrow mesenchymal stromal cells (BMSCs) are the common precursors for both osteoblasts and adipocytes. With aging, BMSC osteoblast differentiation decreases whereas BMSC differentiation into adipocytes increases, resulting in increased adipogenesis and bone loss. In the present study, we investigated the effect of asiatic acid (AA) on adipocytic differentiation of BMSCs. AA inhibited the adipogenic induction of lipid accumulation, activity of glycerol-3-phosphate dehydrogenase, and expression of marker genes in adipogenesis: peroxisome proliferation-activated receptor (PPAR)γ, adipocyte fatty acid-binding protein (ap) 2, and adipsin. Further, we found that AA did not alter clonal expansion rate and expression of C/EBPβ, upstream key regulator of PPARγ, and binding activity of C/EBPβ to PPARγ promoter was not affected by AA as well. These findings suggest that AA may modulate differentiation of BMSCs to cause a lineage shift away from the adipocytes, and inhibition of PPARγ by AA is through C/EBPβ-independent mechanisms. Thus, AA could be a potential candidate for a novel drug against osteoporosis.
- Subjects
MESENCHYMAL stem cells; OSTEOBLASTS; FAT cells; GLYCEROLPHOSPHATE dehydrogenase; ADIPSIN; ADIPOGENESIS
- Publication
Cell Biochemistry & Biophysics, 2014, Vol 68, Issue 2, p437
- ISSN
1085-9195
- Publication type
Article
- DOI
10.1007/s12013-013-9725-2