We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effect of ALA-PDT on inhibition of oral precancerous cell growth and its related mechanisms.
- Authors
Jin, Jian-qiu; Wang, Qian; Zhang, Yu-xing; Wang, Xing; Lu, Zhi-yue; Li, Bo-wen
- Abstract
<bold>Backround: </bold>Early treatment of oral precancerous lesions is considered as a key strategy for in oral carcinogenesis prevention. Increasing evidence has suggested that the transforming growth factor beta (TGF-β) signaling pathway is tightly involved in the process of oral-carcinogenesis. In this study, we investigated the inhibition effect and potential mechanism of 5-aminolaevulinic acid photodynamic therapy (ALA-PDT) in human oral precancerous cells via TGF-β pathway.<bold>Materials and Methods: </bold>Here, the dysplastic oral keratinocyte (DOK) cells were incubated with ALA concentration of 1 mM/mL for 4 h and then irradiated with a Helium-Neon (He-Ne) ion laser at 633 nm (200 mW/cm2). The control cells were cultured in Dulbecco's modified Eagle's medium (DMEM) medium. We analyzed the differentially expressed genes and correlated pathways in oral precancerous cells following ALA-PDT using Affymetrix microarrays. TGF-β pathway was analyzed by quantitative real-time polymerase chain reaction (RT-qPCR) and western blotting. Bioinformatics analysis was performed to evaluate the expression of TGF-β1 in human oral cancer samples and adjacent normal samples. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), flow cytometry, 2'-7'-dichlorodihydrofluorescein diacetate (DCFH-DA), and wound healing assay were used to assess the effects of ALA-PDT plus TGF-β receptor inhibitor (LY2109761) in DOK cells.<bold>Results: </bold>The TGF-β signaling could exert in suppressive effects on DOK cells after ALA-PDT. The cell proliferation and migration rate of DOK cells was significantly reduced and apoptosis and ROS generation induced more effectively by ALA-PDT combined with LY2109761. Furthermore, cell cycle analysis revealed that the combined treatment resulted in G0/G1 phase arrest.<bold>Conclusions: </bold>ALA-PDT suppresses the growth of oral precancerous cells by regulating the TGF-β signaling pathway, and its suppressive effect was enhanced using LY2109761. These results indicate that it could be a promising alternative treatment against oral precancerous lesions.
- Subjects
THERAPEUTIC use of amino acids; PHOTOSENSITIZERS; RESEARCH funding; PRECANCEROUS conditions; CELL physiology; CELL cycle; PHOTOCHEMOTHERAPY; CELL lines; AMINO acids; GROWTH factors; CARCINOGENESIS; PHARMACODYNAMICS
- Publication
Lasers in Medical Science, 2022, Vol 37, Issue 9, p3461
- ISSN
0268-8921
- Publication type
journal article
- DOI
10.1007/s10103-022-03607-y