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- Title
Fluorescence detection in automated DNA sequence analysis.
- Authors
Smith, Lloyd M.; Sanders, Jane Z.; Kaiser, Robert J.; Hughes, Peter; Dodd, Chris; Connell, Charles R.; Heiner, Cheryl; Kent, Stephen B. H.; Hood, Leroy E.
- Abstract
We have developed a method for the partial automation of DNA sequence analysis. Fluorescence detection of the DNA fragments is accomplished by means of a fluorophore covalently attached to the oligonucleotide primer used in enzymatic DNA sequence analysis. A different coloured fluorophore is used for each of the reactions specific for the bases A, C, G and T. The reaction mixtures are combined and co-electrophoresed down a single polyacrylamide gel tube, the separated fluorescent bands of DNA are detected near the bottom of the tube, and the sequence information is acquired directly by computer.
- Publication
Nature, 1986, Vol 321, Issue 6071, p674
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/321674a0