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- Title
Determination of Helicobacter heilmannii in cats by real time polymerase chain reaction and histopathology.
- Authors
Tuzcu, Nevin; Celik, Zeynep; Akcakavak, Gokhan; Bulut, Aysegul; Salık, Rabia; Oner, Muhammed; Kaya, Vedat; Tuzcu, Mehmet
- Abstract
In recent years, many Helicobacter species have been identified in domestic animals and researches on identifying these species as zoonotic agents are increasing. The aim of this study is to reveal the presence of H.heilmannii in stomach and liver tissues taken during necropsies of domestic, stray and shelter cats by comparing the histopathological findings and Real-time PCR results. The material of the study consisted of stomach and liver tissues taken from 41 cats who died from different causes and were necropsied. DNA of H. heilmannii was determined in the stomach tissues of 36 (87.8%) cats and the liver tissues of 24 (58.5%) cats in the study conducted with Real-time PCR using specific primers of H. heilmannii. Histopathologically, degeneration, desquamation and necrosis in the gastric epithelium, fibrosis and edema in the lamina propria, and lymphoplasmatic cell infiltration were detected in cats diagnosed with gastritis. Eight cats were positive in Hematoxylin-Eosin staining and nineteen cats were positive in the staining with Warthin Starry of the sections, in terms of Helicobacter-like microorganisms. Microscopically, dissociation in the remark cords, hydropic degeneration in hepatocytes, and focal mononuclear cell infiltrations in some sections were determined in the livers. In conclusion, the detection of H. heilmannii at the rate of 87.8% in cat stomachs by Real-time PCR revealed a high prevalence of H. heilmannii in cats in the Konya region of Turkey. In addition, it was concluded that histopathological examinations are necessary to correlate the presence of bacteria with the disease state.
- Subjects
HELICOBACTER; REVERSE transcriptase polymerase chain reaction; HISTOPATHOLOGY; EPITHELIUM; FIBROSIS
- Publication
Veterinary Journal of Mehmet Akif Ersoy University, 2023, Vol 8, Issue 2, p69
- ISSN
2148-6239
- Publication type
Article
- DOI
10.24880/maeuvfd.1218001