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- Title
Bacillus sp. AFJ-3 产T-2 毒素降解酶发酵工艺优化.
- Authors
马 妍; 刘虎军; 王 峻; 杜 稳; 孙长坡; 周文化; 赵一凡
- Abstract
The purpose of this study was to increase the enzyme production of T-2 toxin-degrading enzyme produced by bacteria Bacillus sp. AFJ-3, and to reduce the fermentation costs. Using T-2 toxindegrading enzyme activity as an evaluation indicator, the fermentation conditions and media were optimized by single-factor, Plackett-Burman and Box-Behnken test. As a result, the optimal enzymeproducing fermentation process was yeast extract 5 g/L, MgSO4·7H2O 16 g/L, soybean powder 5 g/L, media initial pH 5.8, temperature 36.5 ℃, inoculation 0.5%, and rotation speed 200 r/min. Under this optimized condition, the enzymatic activity of T-2 toxin-degrading enzyme reached 1 674.68 U/mL, which was 47.39% higher than that before optimization. The results provide a reference for the isolation and purification of T-2 toxin-degrading enzymes and the development and application of enzyme preparations.
- Subjects
BACILLUS (Bacteria); BACTERIAL enzymes; YEAST extract; FERMENTATION; ENZYMES
- Publication
Feed Industry, 2023, Vol 44, Issue 19, p95
- ISSN
1001-991X
- Publication type
Article
- DOI
10.13302/j.cnki.fi.2023.19.016