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- Title
Cellular Senescence Biomarker p16INK4a+ Cell Burden in Thigh Adipose is Associated With Poor Physical Function in Older Women.
- Authors
Justice, Jamie N.; Gregory, Heather; Tchkonia, Tamar; LeBrasseur, Nathan K.; Kirkland, James L.; Kritchevsky, Stephen B.; Nicklas, Barbara J.
- Abstract
<bold>Background: </bold>Ample evidence implicates cellular senescence as a contributor to frailty and functional decline in rodents, but considerable effort remains to translate these findings to human aging.<bold>Methods: </bold>We quantified senescence biomarker p16INK4a-expressing cells in thigh adipose tissue obtained from older women previously enrolled in a 5-month resistance training intervention, with or without caloric restriction (RT ± CR, n = 11 baseline, 8 pre-post-intervention pairs). Women in this subsample were older (72.9 ± 3.4 y) and overweight/obese (body mass index: 30.6 ± 2.4 kg/m2). p16INK4a+ cells were identified from 12 to 20 random visual fields/sample at 20× magnification (immunohistochemical, nuclear staining) and were present in all adipose samples.<bold>Results: </bold>Cross-sectional associations were observed between p16INK4a+ cell burden and physical function, including grip strength (r = -0.74), 400-m walk time (r = 0.74), 4-m gait speed (r = -0.73), and self-perceived mobility (r = -0.78) (p ≤ .05). These relationships remained significant after independent adjustments for age and adiposity (p ≤ .05). p16INK4a+ cell abundance was lower following the intervention (pre: 5.47 ± 3.4%, post: 2.17 ± 1.1% count p16INK4a+ cells, p ≤ .05).<bold>Conclusions: </bold>These results provide proof-of-concept that p16INK4a+ cells in thigh adipose are associated with physical function, and may be sensitive to change with RT ± CR in overweight/obese older women.
- Subjects
CELLULAR aging; ADIPOSE tissues; HEALTH of older women; GRIP strength; FUNCTIONAL loss in older people; BIOMARKERS; OVERWEIGHT women; GAIT disorders in old age
- Publication
Journals of Gerontology Series A: Biological Sciences & Medical Sciences, 2018, Vol 73, Issue 7, p939
- ISSN
1079-5006
- Publication type
journal article
- DOI
10.1093/gerona/glx134