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- Title
Post-streptococcal glomerulonephritis: studies on the interaction betwen nephritis strain-associated protein (NSAP), complement and the glomerulus.
- Authors
PEAKE, PHILIP W.; PUSSELL, BRUCE A.; KARPLUS, THOMAS E.; RILEY, ELIZABETH H.; CHARLESWORTH, JOHN A.
- Abstract
Nephritis strain-associated protein (NSAP), a streptokinase produced by strains of streptococci isolated from patients with acute glomerulonephritis, is believed to be a specific antigen which participates in the production of glomerular injury. In order to investigate the mechanisms by which NSAP induces damage we have examined its potential to activate complement in vitro and to bind to isolated human glomeruli. NSAP, both alone and in combination with specific antibody, caused depletion of complement in normal human serum as measured by total haemolytic complement activity and generation of the complement breakdown products, C3a and C4a. Furthermore, Scatchard analysis showed that NSAP bound tightly to human glomeruli (Ka of 400±240 times 106 M) when compared to non-nephritic streptokinase (Ka of 7.3±4.1 times 106 M) and fully cationized human serum albumin (Ka of 0.6±0.04 times 106 M). These findings are consistent with the hypothesis that the deposition of streptococcal antigens within the glomerulus may precede the fixation of complement and specific antibody.
- Publication
APMIS, 1991, Vol 99, Issue 1-6, p460
- ISSN
0903-4641
- Publication type
Article
- DOI
10.1111/j.1699-0463.1991.tb05176.x